Journal: Pharmaceutics

Article Title: Eco-Friendly Synthesis of Copper Oxide Nanoparticles Using Geranium Pelargonium x hortorum Leaf Extract and Its Biological Applications

doi: 10.3390/pharmaceutics17121562

Figure Lengend Snippet: Antimicrobial effect of CuONPs evaluated by agar diffusion against ( a ) S. mutans at 24 h, positive control: chlorhexidine (+); ( b ) C. albicans at 24 h, positive control: amphotericin (+), negative control: sterile water (−). Each value in the graph represents the mean and SD. One-way ANOVA and Tukey’s post hoc test were performed. * Represents a significant difference ( p < 0.05), n = 9 in triplicate experiments.

Article Snippet: The antimicrobial potency of CuONPs was examined using the agar diffusion tests for assessing the antibacterial activity and fungal activity of CuONPs, measuring the diameter of the zone of inhibition (ZOI) of nanoparticles against S. mutans ATCC 25175 bacteria and C. albicans ATCC 90028 fungi.

Techniques: Diffusion-based Assay, Positive Control, Negative Control, Sterility

Journal: Pharmaceutics

Article Title: Eco-Friendly Synthesis of Copper Oxide Nanoparticles Using Geranium Pelargonium x hortorum Leaf Extract and Its Biological Applications

doi: 10.3390/pharmaceutics17121562

Figure Lengend Snippet: Antimicrobial effect of CuONPs by microdilution method to evaluate the growth percentage of S. mutans at 24 h and identify MIC at 235.5 µg/mL −1 and C. albicans at 117.7 µg/mL −1 . Each value on the graph represents the mean and S.D. One-way ANOVA and Tukey post hoc were performed, * representing the concentrations with a significant difference. p < 0.05, n = 9 in duplicate experiments.

Article Snippet: The antimicrobial potency of CuONPs was examined using the agar diffusion tests for assessing the antibacterial activity and fungal activity of CuONPs, measuring the diameter of the zone of inhibition (ZOI) of nanoparticles against S. mutans ATCC 25175 bacteria and C. albicans ATCC 90028 fungi.

Techniques:

Journal: Biomacromolecules

Article Title: Positional Fluorination of Fmoc-Phenylalanine Modulates Hydrogel Structure and Antibacterial Activity

doi: 10.1021/acs.biomac.5c00481

Figure Lengend Snippet: Antibacterial activity of fluorinated Fmoc-Phe hydrogels against S. mutans . (a–c) Bacterial growth kinetics evaluated by turbidity analysis via absorbance readings at 600 nm. S. mutans bacteria were exposed to the hydrogels at various concentrations, as indicated: (a) Fmoc-2-F-Phe, (b) Fmoc-3-F-Phe, and (c) Fmoc-F 5 -Phe. (d–f) Quantification of bacterial viability following 24 h exposure to the various hydrogels at different concentrations measured by luminescence ( n = 5): (d) 0.1 mg/mL, (e) 5 mg/mL, (f) 5 mg/mL compared to 0.1 mg/mL. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 as measured using (d, e) one-way ANOVA, and (f) T-Test.

Article Snippet: S. mutans bacteria (ATCC 35668) were kept at −80 °C.

Techniques: Activity Assay, Bacteria

Journal: Biomacromolecules

Article Title: Positional Fluorination of Fmoc-Phenylalanine Modulates Hydrogel Structure and Antibacterial Activity

doi: 10.1021/acs.biomac.5c00481

Figure Lengend Snippet: Inhibition of S. mutans biofilm formation on fluorinated hydrogels. (a) Schematic representation of biofilm formation and inhibition (a1) biofilm formation and exopolysaccharide detection on an uncoated surface using FITC-conjugated lectin, and (a2) impaired bacterial integrity and reduced biofilm formation on fluorinated Fmoc-Phe hydrogels. (b) Quantification of exopolysaccharides in the biofilm using fluorescence intensity of lectin bound to polysaccharides. (c–l) HRSEM images of S. mutans cultured on the various hydrogels; (c, h) Control, (d, i) Fmoc-2-F-Phe, (e, j) Fmoc-3-F-Phe, (f, k) Fmoc-4-F-Phe, (g, l) Fmoc-F 5 -Phe. Scale Bars are (c–g) 2 μm and (h–l) 1 μm.

Article Snippet: S. mutans bacteria (ATCC 35668) were kept at −80 °C.

Techniques: Inhibition, Fluorescence, Cell Culture, Control